Comparison of two DNA extraction methods for dry Leaf Dipterocarpaceae
نویسندگان
چکیده
DNA extraction is the first step in obtaining high-concentration and high-purity that can use subsequent steps. Because leaf structure challenging contains several secondary metabolites affect results, on dry leaves of Dipterocarpaceae considered problematic. This research aims to find a suitable method for extracting from preserved leaves. Preserved silica gel will up, making them tough destroy. Perform generally using CTAB buffers or kits. According results this study, with modified buffer result higher concentration purity values than extracted kit. isolated be used as template PCR process amplify matK gene.
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ژورنال
عنوان ژورنال: Jurnal Natural
سال: 2022
ISSN: ['2541-4062', '1411-8513']
DOI: https://doi.org/10.24815/jn.v22i3.26588